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液体发酵罐

简要描述:常见的发酵罐分为固体发酵罐和液体发酵罐。发酵罐根据容积和用途不同,分为种子罐(菌种发酵罐)和发酵罐。发酵罐根据需氧类型的不同又分为通风式发酵罐和厌氧发酵罐。

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生物发酵罐Biological Fermentor

发酵罐概述Overview Of Fermentation Tank

常见的发酵罐分为固体发酵罐和液体发酵罐。发酵罐根据容积和用途不同,分为种子罐(菌种发酵罐)和发酵罐。发酵罐根据需氧类型的不同又分为通风式发酵罐和厌氧发酵罐。发酵罐、种子罐,有碳钢制、不锈钢、高镍基合金等,主要用于生化发酵生产。如生产味精、柠檬酸、酶制剂、溶剂、制药、食品等行业。

一、发酵罐的基本条件Basic Conditions Of Fermentation Tank

机械搅拌发酵罐是发酵工厂常用类型之一。它是利用机械搅拌器的作用,使空气和醪液充分混合,促使氧在醪液中溶解,以保证供给微生物生长繁殖、发酵所需要的氧气。使用发酵罐的基本要求:

 

1、发酵罐应具有适宜的径高比。发酵罐的高度与直径之比一般为1.7~4倍左右,罐身越长,氧的利用率较高。

2、发酵罐能承受一定压力。由于发酵罐在消毒及正常工作时,罐内有一定的压力(气压与液压)和温度,因此罐体各部件要有一定的强度,能承受一定的压力。

3、发酵罐的搅拌通风装置使气液充分混合,保证发酵液必须的溶解氧。

4、发酵罐应具有足够的冷却面积。微生物生长代谢过程放出大量的热量,为了控制发酵过程不同阶段所需的温度,应装有足够的冷却面积。

5、发酵罐内应尽量减少死角,避免藏垢积污,易灭菌,避免染菌。

6、搅拌器的轴封应严密,尽量减少泄漏。
 

二、发酵罐罐体尺寸比例Size Proportion Of Fermentation Tank

罐体各部分的尺寸有一定的比例,罐的高度与直径之比一般为1.7~4倍左右。

发酵罐通常装有两组搅拌器,两组搅拌器的间距S约为搅拌器直径的三倍。

对于大型发酵罐以及液体深度HL较高的,可安装三组或三组以上的搅拌器。

最下面一组搅拌器通常与风管出口较接近为好,与罐底的距离C一般等于搅拌器直径Di,但也不宜小于0.8Di,否则会影响液体的循环。

 

三、发酵罐的结构Structure Of Fermentation Tank

好气性机械搅拌发酵罐是密封式受压设备,主要部件包括罐身、搅拌器、轴封、打泡器、联轴器、中间轴承、空气吹泡管(或空气喷射器)、挡板、冷却装置、人孔以及管路等。

系统由发酵罐,空气处理系统、蒸汽净化系统、电器控制系统、恒温系统及管路、阀门、辅助系统,传感器与一次仪表系统,下位机控制系统(现场PLC控制系统,二次仪表),上位机系统以及台架等组成。

 

 

四、液体发酵罐技术参数/Technical Parameter

公称体积

Nominal Volume

5~3000L

装液系数Liquid Loading Coefficient

65-80%, Optimum 70%

罐体配置

Can Body Configuration

不锈钢SUS316L/SUS304材质;
夹套控温,优化导流设计;
内表面抛光,Ra≤0.6μm;外表面抛光或亚光处理;
大视角长条视镜,pH电极、DO电极、温度电极接口齐备;
罐盖配有火焰接种口和补料备用接口等

Stainless steel SUS316L / SUS304 Material;

Jacket temperature control and optimization of diversion design;

Internal surface polishing, RA ≤ 0.6 μ m; External surface polishing or matte treatment (glass shot blasting);

Long strip mirror with large viewing angle, complete interfaces of pH electrode, do electrode and temperature electrode;

The tank cover is equipped with flame inoculation port and feeding standby interface, etc

搅拌系统

Mixing System

顶入式机械搅拌系统;无菌机封系统;直流搅拌电机(10L及以下)或交流电机;
标配2层平直叶轮,一级高效消泡桨;另有斜叶、弯叶、轴向流桨叶等可选;
可调速,数字化设定转速控制

Jacking mechanical mixing system; Aseptic mechanical sealing system; DC mixing motor (10L and below) or AC motor;

Standard configuration: 2-layer flat impeller and one-stage high-efficiency defoaming paddle; There are also inclined blades, curved blades, axial flow blades, etc;

Adjustable speed, digital setting speed control

结构方式

Structure Mode

落地式框架结构,节省空间,万向脚轮,操作方便

Floor type frame structure, space saving, universal casters, convenient operation

通气系统Ventilation System

深层通气,全不锈钢管路;
玻璃转子流量计显示,压力显示,手动阀门调节流量;
配备不锈钢外壳的高效除菌过滤器,过滤精度0.01μm;
需外源压缩空气,配备优质除水稳压装置,持续稳定供气;
可选配质量流量控制器自动控制空气流量

Deep ventilation, all stainless steel pipeline;

Glass rotor flowmeter display, pressure display, manual valve adjustment flow;

High efficiency sterilizing filter equipped with stainless steel housing, with a filtration accuracy of 0.01 μ m;

External compressed air is required, equipped with high-quality water removal and pressure stabilizing devices, to continuously and stably supply gas;

Optional mass flow controller for automatic control of air flow

罐压控制

Tank Pressure Control

顶部排气口配置指针式压力表显示罐压,不锈钢阀门手动调节;
可选配进口压力变送器和自动调节阀实现罐压自动控制

The top exhaust port is equipped with a pointer pressure gauge to display the tank pressure, and the stainless steel valve is manually adjusted;

It can be equipped with inlet pressure transmitter and automatic regulating valve to realize automatic control of tank pressure

温度控制/Temperature Control

在线检测,数字化设定,自动/手动控制自由切换;
德国Pt-100温度探头和数字式温度仪表;
电加热恒温水箱与外源冷却水自动控制温度

Online detection, digital setting, automatic / manual control, free switching;

German PT-100 temperature probe and digital temperature instrument;

Automatic temperature control of electric heating thermostatic water tank and external cooling water

Ph控制

Ph Control

在线检测,1路或2路蠕动泵自动流加碱或酸液进行调节;
进口梅特勒(汉密尔顿)pH电极,可高温蒸汽消毒

Online detection, 1 or 2 peristaltic pumps automatically add alkali or acid for regulation;

Imported Mettler (Hamilton) pH electrode, which can be sterilized by high temperature steam

溶氧(Do)

Dissolved Oxygen (Do)

在线检测,范围0-100%或0-200%;
进口梅特勒(汉密尔顿)DO电极,可高温蒸汽消毒;
可选与转速、通气量联动控制(订购时需明确)

Online detection, range 0-100% or 0-200%;

Imported Mettler (Hamilton) do electrode, which can be sterilized by high temperature steam;

Optional linkage control with speed and ventilation (to be specified when ordering)

补料控制Replenishment Control

标配1路/2路等多路蠕动泵时间比例流加补料,流加量累积显示、记录

It is equipped with 1-way / 2-way and other multi-channel peristaltic pumps as standard, with time proportional flow feeding and feeding, and the cumulative flow feeding amount is displayed and recorded

消泡方式Defoaming Mode

传导式泡沫电极,泡沫异常状况自动报警;
标配1路蠕动泵自动/手动流加消泡剂,流加量累积显示、记录

The conductive foam electrode automatically alarm the abnormal condition of foam.

One peristaltic pump is equipped as standard for automatic / manual flow of defoamer, and the cumulative flow dosage is displayed and recorded

控制系统

Control System

应用工业级MC-bio控制系统,采用西门子PLC控制器;
32位真彩色触摸屏操作界面,功能齐全、运行稳定;
另有A型简易型分仪表控制系统可选

Apply industrial MC bio control system and Siemens PLC controller;

32-bit true color touch screen operation interface with complete functions and stable operation;

Another type a simple instrument control system is optional

灭菌方式/Sterilization Method

在位灭菌,手动控制,需接外源饱和蒸汽

In place sterilization, manual control, need to connect external saturated steam

可选配项/Optional Configuration

1.多路补料 Multiple feeding
2.全自动灭菌 Automatic sterilization(AUTO-SIP)
3.补料称重 Feeding and weighing
4.罐体称重 Tank weighing
5.富氧旁路 Oxygen enriched bypass
6.氧化还原电位在线检测 On line detection of redox potential
7.空气流量自动检测与控制 Automatic detection and control of air flow
8.尾气CH4,O2、CO2含量在线检测 Online detection of CH4, O2 and CO2 content in tail gas
9.甲醇(乙醇)含量在线检测 Online detection of methanol (ethanol) content
10.葡萄糖、特定氨基酸在线检测 On line detection of glucose and specific amino acids

设备特点Equipment Characteristics

在位灭菌,安全可靠,培养基浓度准确;
无传动泄漏风险;
大视角纵向视镜观测清晰;
接种方式多样可靠(标配火焰接种口,差压接种口等多种形式可选);
落地式罐型美观大方;
操作拆装容易;
特殊规格可以定制。

In situ sterilization, safe and reliable, accurate medium concentration;

No risk of transmission leakage;

Large viewing angle longitudinal mirror observation is clear;

Various and reliable inoculation methods (standard flame inoculation port, differential pressure inoculation port and other forms are optional);

Floor type tank, beautiful and generous;

Easy operation and disassembly;

Special specifications can be customized.

 

 

 

 

 

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